Some parameters of human physiological responses serve as markers of eco-friendly and comfortable living. Given that 50% of the world's population lives in cities, the study of the impact of the urban environment on humans is of high relevance. The urban visual environment, which can be comfortable or uncomfortable, is an important environmental factor. We studied features of eye-tracking, brain activity and responses of the autonomic nervous system during perception of urban environment images of different comfort. Participants included 50 healthy people of both sexes aged 21. It was revealed that while viewing modern monochrome minimalistic and homogeneous urban space, the number of saccades increased, the duration of saccades and fixations decreased. This eye-tracking corresponds to the work of the human visual system in search mode. At the same time, the intense work of the visual system is accompanied by the flow of absolutely identical information to the brain. This changes the scenario of evolutionarily conserved brain mechanisms of visual stimuli processing. Bioelectric activity of the cerebral cortex is reduced even in areas that are specialized for this activity. The activity of subcortical structures of the brain responsible for the formation of the emotional component increases. Furthermore, we recorded activation of the ergotropic system of the brain, that favors the mobilization of the body reserves. Such physiological changes are also typical for stress response of the body. Spending a long time in a visually uncomfortable environment leads to chronic stress and the development of pathologies