Types of presentation
Oral Presentation
Presentation time for ORAL presentation is 10 minutes + 5 min for questions from the audience.
You shall make your presentation in a timely manner. Kindly note that the time is usually followed the the conference Chairman.
All presentations have to be made in English
Speakers are kindly asked to use the available in the Conference hall Laptop. Only if they have special reasons they may use their own laptops (in that case they have to inform the technical assistant before the session).
Only PowerPoint presentations on a CD-ROM/DVD or a USB Memory stick will be accepted.
Types of presentation
Oral Presentation
Presentation time for ORAL presentation is 10 minutes + 5 min for questions from the audience.
You shall make your presentation in a timely manner. Kindly note that the time is usually followed the the conference Chairman.
All presentations have to be made in English
Speakers are kindly asked to use the available in the Conference hall Laptop. Only if they have special reasons they may use their own laptops (in that case they have to inform the technical assistant before the session).
Only PowerPoint presentations on a CD-ROM/DVD or a USB Memory stick will be accepted.
Types of presentation
Oral Presentation
Presentation time for ORAL presentation is 10 minutes + 5 min for questions from the audience.
You shall make your presentation in a timely manner. Kindly note that the time is usually followed the the conference Chairman.
All presentations have to be made in English
Speakers are kindly asked to use the available in the Conference hall Laptop. Only if they have special reasons they may use their own laptops (in that case they have to inform the technical assistant before the session).
Only PowerPoint presentations on a CD-ROM/DVD or a USB Memory stick will be accepted.
Types of presentation
Oral Presentation
Presentation time for ORAL presentation is 10 minutes + 5 min for questions from the audience.
You shall make your presentation in a timely manner. Kindly note that the time is usually followed the the conference Chairman.
All presentations have to be made in English
Speakers are kindly asked to use the available in the Conference hall Laptop. Only if they have special reasons they may use their own laptops (in that case they have to inform the technical assistant before the session).
Only PowerPoint presentations on a CD-ROM/DVD or a USB Memory stick will be accepted.
Publication ethics
Authors
The Author declares that the manuscript is original and in its present form has not been published elsewhere in any form, that it has not been submitted to any journal/proceedings and that it will not be submitted to any other journal/proceedings, if it is accepted for publication in the GEOLINKS Conference Proceedings.
The Author is responsible for the research they have been done and for the results described in the paper.
The Author warrants that the article doesn't infringe upon any copyright, contains no libelous or otherwise unlawful statements, and does not otherwise infringe on the rights of others.
The Author transfers the copyright for this publication/article to the GEOLINKS Conferences.
The Author licenses to the Publisher the right to distribute the Article as a part of GEOLINKS Conference proceedings.
Submission Guidelines
Abstract Submission
An abstract between 300 to 500 words, clearly summarising the arguments, should be submitted before deadline.
The preferred length of paper (including footnotes) is 5,000 words (maximum limit). In general, please confine your paper between 10-12 pages, everything included.
It is author's responsibility to ensure that all references and citations are correct, and that the contribution does not contain any material that infringes copyright, or is defamatory, obscene or otherwise unlawful or litigious. Please check GEOLINKS Tips.
Copyright in the article will remain jointly with the owner of the copyright and the Publisher. By submitting an article to the conference, the owner of the copyright grants the publisher with a license to publish the article. The author warrants that he is the owner of all rights of copyright in the article. Where the author subsequently publishes the article, the author is requested to acknowledge the article appeared in the conference proceedings.
The Author(s) will indemnify and defend the Publisher against any claim, demand or recovery against the Publisher by reason of any violation of any proprietary right or copyright, or because of any libellous or scandalous matter contained in the Manuscript.
The Publisher will have the right to edit the work for the original edition and for any revision, provided that the meaning of the text is not materially altered.
Submission Guidelines
Abstract Submission
An abstract between 300 to 500 words, clearly summarising the arguments, should be submitted before deadline.
The preferred length of paper (including footnotes) is 5,000 words (maximum limit). In general, please confine your paper between 10-12 pages, everything included.
It is author's responsibility to ensure that all references and citations are correct, and that the contribution does not contain any material that infringes copyright, or is defamatory, obscene or otherwise unlawful or litigious. Please check GEOLINKS Tips.
Copyright in the article will remain jointly with the owner of the copyright and the Publisher. By submitting an article to the conference, the owner of the copyright grants the publisher with a license to publish the article. The author warrants that he is the owner of all rights of copyright in the article. Where the author subsequently publishes the article, the author is requested to acknowledge the article appeared in the conference proceedings.
The Author(s) will indemnify and defend the Publisher against any claim, demand or recovery against the Publisher by reason of any violation of any proprietary right or copyright, or because of any libellous or scandalous matter contained in the Manuscript.
The Publisher will have the right to edit the work for the original edition and for any revision, provided that the meaning of the text is not materially altered.
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Proceedings 2020
All GEOLINKS 2020 participants get an online access to the full conference proceedings.
You can find the full digital books, including all accepted and published double blind peer-reviewed papers.

GEOLINKS Proceedings
Book 1 Volume 2
Air Pollution and Climate Change
Biotechnologies
Environmental Geology
Soil Science
Water Resources
GEOLINKS Proceedings
Book 2 Volume 2
Ecology and Environmental Studies
Environmental Economics
Green Buildings Technology and Materials
Green Design and Sustainable Architecture
GEOLINKS International Conference 2021, Book 1
BIOTECHNOLOGIES
OBTAINING NUCLEIC ACID PREPARATIONS AND THEIR HYDROLYSATES FROM BIOMASS OF METHANE-OXIDIZING BACTERIA
Prof. Dr. Alla A. Krasnoshtanova, Elisaveta K. Borovkova
ABSTRACT
Due to the unfavourable environmental, social and economic situation, the need for the treatment of oncological diseases and diseases associated with impaired activity of the immune system is increasing. A lot of these drugs are made on the basis of nucleic acid components, the industrial production of which is practically non-existent in Russia. Therefore, a task of current interest is to develop the basis of the technology for obtaining components of nucleic acids, which can be widely used in medicine as immunomodulatory, wound-healing, antiviral, and diagnostic medicine, as well as for cancer treatment.
Most of the described in literature methods of isolating nucleic acid components from plant, animal and microbial raw materials are based on the use of toxic and expensive organic solvents, that’s why it is impossible to apply these methods outside of laboratory conditions. The most promising source of raw materials for nucleic acids is the biomass of microorganisms (yeast and bacteria) from biomass, since the use of such source makes it possible to quickly obtain a large enough amount of biomass, and, consequently, a larger amount of nucleic acids. This allows obtaining DNA in addition to RNA. RNA and DNA substances can be used to obtain nucleosides and nitrogenous bases, which are also widely used in medicine.
The purpose of these studies was to select the conditions for the extraction of RNA and DNA from the biomass of methane-oxidizing bacteria in one technological cycle, as well as to compare the efficiency of alkaline and acid hydrolysis of microbial RNA and DNA. The need for a two-stage extraction of nucleic acids from the biomass of methane-oxidizing bacteria in order to separately extract RNA and DNA was Substantiated. It was ascertained that at the first stage of extraction at a temperature of 90 ° C, pH 9.0 for 90 min, at least 85% of RNA is extracted. After the separation of the extract by centrifugation, the partially denuclearized biomass must be re-processed under the same conditions in order to extract DNA by at least 83%. The modes of concentration of RNA and DNA solutions by ultrafiltration were selected. It was found that in order to achieve effective deposition of nucleic acids at the isoelectric point, the concentration of the RNA solution must be carried out on the UPM-10 membrane at the concentration degree of 7, and the DNA solution on the UPM-100 membrane at the concentration degree 6. The dynamics of decomposition of nucleic-protein complexes in the medium of monoammonium phosphate was investigated. It was shown that the transition of NA into solution by at least 80% is achieved at a monoammonium phosphate concentration of 1.7 M, a temperature of 55 ° C for 90 min. The use of 5-fold washing of oligonucleotide substances with acidified water (pH 2.0) to remove excess mineral impurities was substantiated. А comparative assessment of acid and alkaline hydrolysis of RNA and DNA was carried out in order to obtain derivatives of nucleic acids.
KEYWORDS
methane-oxidizing bacteria, RNA, DNA, acid hydrolysis, alkaline hydrolysis