Types of presentation
Oral Presentation
Presentation time for ORAL presentation is 10 minutes + 5 min for questions from the audience.
You shall make your presentation in a timely manner. Kindly note that the time is usually followed the the conference Chairman.
All presentations have to be made in English
Speakers are kindly asked to use the available in the Conference hall Laptop. Only if they have special reasons they may use their own laptops (in that case they have to inform the technical assistant before the session).
Only PowerPoint presentations on a CD-ROM/DVD or a USB Memory stick will be accepted.
Types of presentation
Oral Presentation
Presentation time for ORAL presentation is 10 minutes + 5 min for questions from the audience.
You shall make your presentation in a timely manner. Kindly note that the time is usually followed the the conference Chairman.
All presentations have to be made in English
Speakers are kindly asked to use the available in the Conference hall Laptop. Only if they have special reasons they may use their own laptops (in that case they have to inform the technical assistant before the session).
Only PowerPoint presentations on a CD-ROM/DVD or a USB Memory stick will be accepted.
Types of presentation
Oral Presentation
Presentation time for ORAL presentation is 10 minutes + 5 min for questions from the audience.
You shall make your presentation in a timely manner. Kindly note that the time is usually followed the the conference Chairman.
All presentations have to be made in English
Speakers are kindly asked to use the available in the Conference hall Laptop. Only if they have special reasons they may use their own laptops (in that case they have to inform the technical assistant before the session).
Only PowerPoint presentations on a CD-ROM/DVD or a USB Memory stick will be accepted.
Types of presentation
Oral Presentation
Presentation time for ORAL presentation is 10 minutes + 5 min for questions from the audience.
You shall make your presentation in a timely manner. Kindly note that the time is usually followed the the conference Chairman.
All presentations have to be made in English
Speakers are kindly asked to use the available in the Conference hall Laptop. Only if they have special reasons they may use their own laptops (in that case they have to inform the technical assistant before the session).
Only PowerPoint presentations on a CD-ROM/DVD or a USB Memory stick will be accepted.
Publication ethics
Authors
The Author declares that the manuscript is original and in its present form has not been published elsewhere in any form, that it has not been submitted to any journal/proceedings and that it will not be submitted to any other journal/proceedings, if it is accepted for publication in the GEOLINKS Conference Proceedings.
The Author is responsible for the research they have been done and for the results described in the paper.
The Author warrants that the article doesn't infringe upon any copyright, contains no libelous or otherwise unlawful statements, and does not otherwise infringe on the rights of others.
The Author transfers the copyright for this publication/article to the GEOLINKS Conferences.
The Author licenses to the Publisher the right to distribute the Article as a part of GEOLINKS Conference proceedings.
Submission Guidelines
Abstract Submission
An abstract between 300 to 500 words, clearly summarising the arguments, should be submitted before deadline.
The preferred length of paper (including footnotes) is 5,000 words (maximum limit). In general, please confine your paper between 10-12 pages, everything included.
It is author's responsibility to ensure that all references and citations are correct, and that the contribution does not contain any material that infringes copyright, or is defamatory, obscene or otherwise unlawful or litigious. Please check GEOLINKS Tips.
Copyright in the article will remain jointly with the owner of the copyright and the Publisher. By submitting an article to the conference, the owner of the copyright grants the publisher with a license to publish the article. The author warrants that he is the owner of all rights of copyright in the article. Where the author subsequently publishes the article, the author is requested to acknowledge the article appeared in the conference proceedings.
The Author(s) will indemnify and defend the Publisher against any claim, demand or recovery against the Publisher by reason of any violation of any proprietary right or copyright, or because of any libellous or scandalous matter contained in the Manuscript.
The Publisher will have the right to edit the work for the original edition and for any revision, provided that the meaning of the text is not materially altered.
Submission Guidelines
Abstract Submission
An abstract between 300 to 500 words, clearly summarising the arguments, should be submitted before deadline.
The preferred length of paper (including footnotes) is 5,000 words (maximum limit). In general, please confine your paper between 10-12 pages, everything included.
It is author's responsibility to ensure that all references and citations are correct, and that the contribution does not contain any material that infringes copyright, or is defamatory, obscene or otherwise unlawful or litigious. Please check GEOLINKS Tips.
Copyright in the article will remain jointly with the owner of the copyright and the Publisher. By submitting an article to the conference, the owner of the copyright grants the publisher with a license to publish the article. The author warrants that he is the owner of all rights of copyright in the article. Where the author subsequently publishes the article, the author is requested to acknowledge the article appeared in the conference proceedings.
The Author(s) will indemnify and defend the Publisher against any claim, demand or recovery against the Publisher by reason of any violation of any proprietary right or copyright, or because of any libellous or scandalous matter contained in the Manuscript.
The Publisher will have the right to edit the work for the original edition and for any revision, provided that the meaning of the text is not materially altered.
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Types of presentation
Proceedings 2020
All GEOLINKS 2020 participants get an online access to the full conference proceedings.
You can find the full digital books, including all accepted and published double blind peer-reviewed papers.

GEOLINKS Proceedings
Book 1 Volume 2
Air Pollution and Climate Change
Biotechnologies
Environmental Geology
Soil Science
Water Resources
GEOLINKS Proceedings
Book 2 Volume 2
Ecology and Environmental Studies
Environmental Economics
Green Buildings Technology and Materials
Green Design and Sustainable Architecture
GEOLINKS International Conference 2021, Book 1
BIOTECHNOLOGIES
PRODUCTION OF ANTIBODIES FROM POULTRY YOLK (IgY) AND INVESTIGATION OF THEIR IMMUNOCHEMICAL PROPERTIES
Prof. Dr. Alla A. Krasnoshtanova, Alesya N. Yudina
ABSTRACT
A particularly important aspect of immunology is to develop non-invasive methods of obtaining antibodies which could be a great alternative to traditional ones that based on the harmful procedure of isolation of immunoglobulins from animal blood sera. That’s why the extraction of antibodies from poultry egg yolks (IgY) is the most promising. Due to the fact of variation of IgY structural features that determine the definite immunochemical properties, yolk antibodies in comparison with mammalian immunoglobulins (IgG) does not interact with rheumatoid factor (Rf), contribute to the activation of the complement system, bind to the Fc-receptor (FcR), and also has weak cross-reactivity, which confirms the possibility of their widespread use in medicine and food. Also the presence of phylogenetic distance between chickens and mammalians guarantees immune response against conservative mammalian protein molecules which is highly important for the creation of new generation test systems.
The aim of this work is to develop a selective method of producing high-purity immunoglobulin Y preparations from the yolk of chicken eggs.
There were adopted selective conditions of isolation of IgY under spontaneous thawing procedure at the room temperature of firstly frozen yolk solution in a sodium-phosphate buffer mixed with water (pH 5.0) in a ratio of 1:6, which leads to receiving a water-soluble fraction further precipitated with the sodium chloride at a concentration of 10% of the solution mass and subsequently concentrated using ultrafiltration with membrane UAM-10, that allows achieving the content of IgY not less than 95% per dry substance in immunoglobulin fraction. It is possible to produce a protein fraction with a protein content of at least 9 g/l.
The purity of the immunoglobulin fraction was verified using polyacrylamide gel electrophoresis. The presence of a light chain in the IgY solution was proved to be a low-molecular compound using the method of gel-filtration-chromatography. The immunological activity of IgY was studied with respect to bovine serum albumin (BSA) as an antigen. The enzymatic resistance of IgY against proteolytic enzymes was tested in area of the gastrointestinal tract.
KEYWORDS
egg yolk, IgY, immune response, gastrointestinal enzymes, ultrafiltration